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KMID : 0603820100160040259
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Journal of Experimental & Biomedical Science
2010 Volume.16 No. 4 p.259 ~ p.264
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Effects of Nitric Oxide on the Neuronal Activity of Rat Cerebellar Purkinje Neurons
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Jang Su-Jeong
Jeong Han-Seong
Park Jong-Seong
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Abstract
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This study was designed to investigate the effects of nitric oxide on the neuronal activity of rat cerebellar Purkinje cells. Sprague-Dawley rats aged 14 to 16 days were decapitated under ether anesthesia. After treatment with pronase and thermolysin, the dissociated Purkinje cells were transferred into a chamber on an inverted microscope. Spontaneous action potentials and potassium currents were recorded by standard patch-clamp techniques under current and voltageclamp modes respectively. 15 Purkinje cells revealed excitatory responses to 20 μM of sodium nitroprusside (SNP) and 4 neurons (20%) did not respond to SNP. Whole potassium currents of Purkinje cells were decreased by SNP (n=10). Whole potassium currents of Purkinje cells were also decreased by L-arginine, substrate of nitric oxide (n=10). These experimental results suggest that nitric oxide increases the neuronal activity of Purkinje cells by altering the resting membrane potential and afterhyperpolarization.
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KEYWORD
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Nitric oxide, Purkinje cell, L-arginine, Neuronal activity
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